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The study investigated chemistry students’ knowledge and practices of chemical waste management in chemistry laboratories in a higher educational institution in Ghana. Descriptive survey design was employed, and the stratified sample comprised of 93 chemistry major students who were selected from levels 100, 200 and 300 in ¾ÅÉ«ÊÓƵ. Questionnaires and unstructured interview were used to collect data which were then analyzed by descriptive statistics (mean and standard deviation) and inferential statistics (t-test and correlation). The results revealed students’ good knowledge of, yet poor practices of chemical waste management in the various chemistry laboratories. The t-test conducted revealed a statistically significant difference (p=0.000<0.05) in students’ knowledge and practices of chemical waste management in the school’s chemistry laboratories. Also, a Pearson correlation (r=0.415) corroborated the finding that students weakly put their good knowledge in chemical waste management into practice. It is recommended for tertiary educational institutions to build a culture of sustainability and environmental protection in accordance with Sustainable Development Goals (SDG 6 and SDG 11) [3] among science students to by encouraging them to put their knowledge about waste management into practice and protect the environment.