In our world today, the control over and the use of a country’s natural resources (and the biological diversity of which they are a part) usually present a lot of challenges for both policy makers and implementing agencies and institutions. These challenges range from weak institutional capacities and technocratic hurdles to opposition from local communities for whom policies may be meant for. However, if such challenges are effectively mitigated, large prospects usually associated with the sustainable use and management of these natural resources may be realised. In this article, based on intensive interview of experts and critical review of official reports and policy documents, we identify a number of challenges associated the implementation of the Convention on Biological Diversity (CBD) in Ghana and recommend ways of addressing these challenges. The study finds that there is usually a wide knowledge and information gap on issues related to biodiversity in Ghana. Moreover, there is inadequate funding which also leads to the inability to retain relevant experts. In addition, there is the complex nature of implementing multilateral environmental agreements in Ghana and the lack of adequate publicity on the essence of the CBD. Key among the recommendations we make are effectively engaging civil society organisations on issues of biodiversity conservation and sustainable development; the enhancement of Alternative Livelihood Projects (EnALPs); stringent enforcement of punitive and preventive measures and; the implementation of finance-generating biodiversity services.