Although Big Data Analytic Capability (BDAC) has advanced in some organisations, the impact on collaboration and business innovation is unnoticed in the literature. Therefore, this study examines the influence of BDAC on Collaboration Business Culture (CBC) and business innovation. The study further investigates how the relationships between CBC and business innovation and BDAC and CBC are mediated and moderated, respectively. Data was collected from 577 managers in selected organisations using a survey questionnaire. The research hypotheses were examined using a PLS-SEM. The study reveals that BDAC positively impacts CBC and business innovation, while CBC influences business innovation. The result also indicates that BDAC partially mediates the CBC and innovation relationship. The findings also revealed that BDAC moderates the CBC and business innovation relationship. Detailed knowledge contributions and managerial implications are discussed